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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human CLDN5 protein
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
Cytometric bead array, Indirect ELISA
- 靶点:
Claudin 5
- 抗体英文名:
Claudin 5 Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名:
Claudin 5 Recombinant monoclonal antibody, PBS Only (Detector)
- 规格:
100ug
产品信息
83046-3-PBS targets Claudin 5 as part of a matched antibody pair:
MP00128-2: 83046-1-PBS capture and 83046-3-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag29044 Product name: Recombinant human CLDN5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 144-218 aa of BC032363 Sequence: VREFYDPSVPVSQKYELGAALYIGWAATALLMVGGCLLCCGAWVCTGRPDLSFPVKYSAPRRPTATGDYDKKNYV |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | claudin 5 |
| 别名 | CLDN5, AWAL, BEC1, Claudin5, Claudin-5 |
| 计算分子量 | 218 aa, 23 kDa |
| GenBank蛋白编号 | BC032363 |
| 基因名称 | CLDN5 |
| Gene ID (NCBI) | 7122 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O00501 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验50μL。 封闭液:用PBS将试剂盒中的封闭存储液(Blocking stock solution R)稀释10倍,每孔200μL。 抗体稀释液:注意,只是用来稀释检测抗体和酶联亲和素。用PBS将试剂盒中的稀释存储液(Dilution buffer R)稀释10倍 检测抗体(Biotinylated detector antibody,Secondary antibody):照U-Cytech说明书,加入双蒸水溶解。使用时,用抗体稀释液稀释100倍,每孔100μL。 酶联亲和
以非那西丁为探针采用高效液相色谱法快速测定细胞色素P450 1A2的酶活性
acid). The flow rate was 2.0 ml・min-1, and the UV detector was set at 244 nm. Firstly, phenacetin was incubated with human CYP 450 1A2 enzyme in vitro at 37℃ and stopped by addition of 50 μl acetonitrile and centrifuged (10 000 g) for 3 min. Finally
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