- ￥1100
- Proteintech
- 65120-1-Ig
- 2025年10月09日
- FC
- Armenian Hamster
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Mouse
- 宿主：
Armenian Hamster
- 应用范围：
FC
- 靶点：
PD-1/CD279
- 抗体英文名：
Anti-Mouse PD-1/CD279 (J43.1)
- 抗体名：
Anti-Mouse PD-1/CD279 (J43.1)
- 规格：
500ug
经过测试的应用
| Positive FC detected in | Anti-CD3/CD28 stimulated mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
65120-1-Ig targets PD-1/CD279 in FC applications and shows reactivity with Mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | Mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Armenian Hamster / IgG |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | programmed cell death 1 |
| 别名 | CD279, Ly101, PD 1, PD-1, Pdc1, Pdcd1, programmed cell death 1 |
| GenBank蛋白编号 | BC119179 |
| 基因名称 | PD-1 |
| Gene ID (NCBI) | 18566 |
| RRID | AB_3670274 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | Q02242 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).
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文献和实验(hematopoietic stem cells) 和CD279 (PD-1)。 · 将孵育好的样品加入到 1 ml 的 Strep-Tactin®TACS 琼脂糖层析柱上（柱子最大结合能力：1 x 108个细胞）。 · 当样品完全进入柱子后，加入 10 ml Buffer洗脱两次，就可以得到未结合的细胞了。后续使用流式细胞术检测富集的 NK 细胞。 （此处用到的试剂见文末） 结果与讨论 通过使用阴选将非目的细胞去除后，获得了高纯度的目的细胞群。实验用到了 Strep-Tactin®TACS 琼脂糖层析
网络 二、Ig超家族的特点 （一）Ig超家族的结构特点 Ig超家族成员均含有1～7个Ig样结构域，每个Ig样结构域约含70～110个氨基酸残基。其二级结构是两个各含3～5个反平行β折叠股所形成的β片层（anti-parallel β-pleated sheet)平面，每个反平行β折叠股由5～10个氨基酸残基组成，β
Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE
to anaphylaxis. Here, we describe the history behind the IgE discovery and the protocol for purifying IgE and anti-IgE in the mouse. This is followed by our ELISA protocol for mouse IgE detection.
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