- ￥5800
- Proteintech
- 84605-4-PBS
- 2025年10月09日
- WB, Indirect ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PZP protein
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, Indirect ELISA
- 靶点：
Pregnancy Zone Protein
- 抗体英文名：
Pregnancy Zone Protein Recombinant monoclonal antibody, PBS Only
- 抗体名：
Pregnancy Zone Protein Recombinant monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
84605-4-PBS targets Pregnancy Zone Protein in WB, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag16471 Product name: Recombinant human PZP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 341-690 aa of BC111756 Sequence: ITAHYTLNRQAMGELSELSFHYLIMAKGVIVRSGTHTLPVESGDMKGSFALSFPVESDVAPIARMFIFAILPDGEVVGDSEKFEIENCLANKVDLSFSPAQSPPASHAHLQVAAAPQSLCALRAVDQSVLLMKPEAELSVSSVYNLLTVKDLTNFPDNVDQQEEEQGHCPRPFFIHNGAIYVPLSSNEADIYSFLKGMGLKVFTNSKIRKPKSCSVIPSVSAGAVGQGYYGAGLGVVERPYVPQLGTYNVIPLNNEQSSGPVPETVRSYFPETWIWELVAVNSSGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTMPYSVIRGEVFTLKATVL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | pregnancy-zone protein |
| 别名 | PZP, pregnancy-zone protein, 242114A6 |
| 计算分子量 | 1482 aa, 164 kDa |
| 观测分子量 | 180 kDa |
| GenBank蛋白编号 | BC111756 |
| 基因名称 | Pregnancy Zone Protein |
| Gene ID (NCBI) | 5858 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P20742 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Pregnancy Zone Protein (PZP) is an immunosuppressive protein that is expressed by the placenta. The PZP is a thiol ester-containing alpha macroglobulin protein that controls the proteolytic pathways during pregnancy and contributes to maintaining the immunologically privileged status of the fetus. Recently PZP over-expression has been described as a novel biomarker for predicting the prognosis of hepatocellular carcinoma and its high expression has been reported in solid tumors.
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文献和实验少可以参考文献上给定的硫酸铵固体的溶解度表，《Guide to Protein Purification》一书中就有详细的溶解度表，静置两个小时左右，10000 g离心半个小时后，弃去上清，用PBS重新溶解沉淀，然后再次加入硫酸铵固体至45%饱和，静置两个小时左右10000 g离心半个小时，弃去上清，用少量PBS溶解沉淀，即得到较纯的抗体，因为此抗体中尚有较高浓度的硫酸铵，因此需要对PBS透析，一般透析一天一夜较合适，透析过程中更换透析液两至三次。如果对纯度要求不是很高，也可以不做第一次沉淀，直接用45
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