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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
mouse
- 宿主:
Rabbit
- 应用范围:
Cytometric bead array, Indirect ELISA
- 靶点:
IL-27A
- 抗体英文名:
Mouse IL-27A Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名:
Mouse IL-27A Recombinant monoclonal antibody, PBS Only (Detector)
- 规格:
100ug
产品信息
85795-2-PBS targets IL-27A as part of a matched antibody pair:
MP02119-1: 85795-3-PBS capture and 85795-2-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 27 |
| 别名 | 242655D10 |
| 基因名称 | Il27 |
| Gene ID (NCBI) | 246779 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O35228(IL27B)+Q8K3I6(IL27A) |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验我们知道,PeproTech的所有细胞因子和蛋白均为冻干粉,这使得运输非常便捷,只要常温即可。而且,细胞因子和蛋白冻干粉非常稳定,在-20℃或-80℃条件下可保存数年。冻干粉在使用前需进行溶解,然后以液体形式加到培养体系或注射入动物体内。溶解步骤非常关键,因溶解不好会导致细胞因子或蛋白的失活,这也是很多用户在实际使用中经常遇到的问题。 那么,应该如何进行正确的溶解呢? 下面我们以Recombinant Human IL-4 (重组人IL-4,产品编号:200-04)的说明书为例
Th17 Polarization of Mouse CD4 Cells
实验试剂 Sterile PBS Cell culture medium Sterile plastic petridishes RBC Lysis Buffer [Details: Biolengend, cat.#420301] Anti-mouse CD3ε, clone145-2C11[Details: LEAF™format, cat.#100314]
我们以Recombinant Human IL-4 (重组 人IL-4,产品编号:200-04)的说明书为例,对细胞因子或蛋白的溶解方法进行详细的阐述。 拿到重组 人IL-4的说明书后,您会发现有一段关于Reconstitution(重悬)的叙述,这段内容含有溶解相关的所有信息。 1. Centrifuge the vial prior to opening 第 1 步:开盖前离心试剂管 PeproTech的细胞因子 或蛋白冻干粉装盛在塑料管中,为无菌包装。冻干
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