- ￥800 - 1990
- Proteintech
- CL750-65214
- 2025年10月09日
- FC
- Rat
- Mouse
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
Mouse
- 宿主：
Rat
- 应用范围：
FC
- 靶点：
PLVAP
- 抗体英文名：
CoraLite® Plus 750 Anti-Mouse PLVAP (MECA-32)
- 抗体名：
CoraLite® Plus 750 Anti-Mouse PLVAP (MECA-32)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥800.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1990.0 |
经过测试的应用
| Positive FC detected in | bEnd.3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65214 targets PLVAP in FC applications and shows reactivity with Mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | Mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | plasmalemma vesicle associated protein |
| 别名 | MECA32, Plvap, PV 1 |
| GenBank蛋白编号 | BC013517 |
| 基因名称 | Plvap |
| Gene ID (NCBI) | 84094 |
| RRID | AB_3084869 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q91VC4 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Screening Colonies by Hybridization with Radiolabeled Probe
: 4.5 ml 20 x SSC stock, 750 µl 1 x BLOTTO, 9.75 ml dH2 O for 30 ml: 9 ml 20 x SSC stock, 1.5 ml 1 x BLOTTO, 19.5 ml dH2 O for 100 ml: 30 ml 20 x SSC stock, 5 ml 1 x BLOTTO, 65 ml dH2 O 32 P-labeled probe (~ 1 mio cpm
经750g、离心10min后留上清，以9000g离心20min后留沉淀，重新悬浮后以9000g再离心20min，弃上清得线粒体.全部操作于4℃进行。 (2)培养细胞提取线粒体。细胞弃去培养液后，用细胞收集液作用2到3分钟，用scraper，将细胞收集起来，6000g，离心15分钟。弃上清，之后加细胞匀浆液，用匀浆器或手动匀浆管匀浆，将装有匀浆液的离心管配平后放入普通离心机，以2500rpm，离心15分钟，缓缓取上清液，移入高速离心管中，保存于有冰块的烧杯中。以17000rpm离心20分钟，弃上清
技术资料暂无技术资料 索取技术资料
