- ￥810 - 2020
- Proteintech
- CL750-65139
- 2025年10月09日
- FC
- Rat
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
mouse
- 宿主：
Rat
- 应用范围：
FC
- 靶点：
CD45R (B220)
- 抗体英文名：
CoraLite® Plus 750 Anti-Mouse CD45R (B220) (RA3-6B2)
- 抗体名：
CoraLite® Plus 750 Anti-Mouse CD45R (B220) (RA3-6B2)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥810.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥2020.0 |
经过测试的应用
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65139 targets CD45R (B220) in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, C |
| 别名 | B220, CD45R, Cd45, Ptprc, EC:3.1.3.48 |
| GenBank蛋白编号 | BC028512 |
| 基因名称 | CD45 |
| Gene ID (NCBI) | 19264 |
| RRID | AB_2924095 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P06800 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.
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文献和实验Intracellular Cytokine Staining Protocol
CD45R/B220 RA3-6B2 14-0452 also on NK progenitors, LAK, activated T Natural Killer Cells (NK) CD49b/DX5 DX5 14-5971
Intracellular Cytokine Staining Protocol
pre- to mature B, not on plasma 6D5 14-0192 CD45R/B220 RA3-6B2 14-0452 also on NK progenitors, LAK, activated T
Fluorochrome Absorption and Emission Spectra
?10-nm bandpass filter will give optimum detection for this fluorochrome (Fig. 1). Comparative staining using a monoclonal antibody (RA3-6B2; anti-B220; Cat. No. 557390/553084**) conjugated
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