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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human IBA1 protein
- 亚型:
IgG1
- 保存条件:
Store at -80°C.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Mouse
- 应用范围:
Cytometric bead array, Indirect ELISA
- 靶点:
IBA1
- 抗体英文名:
IBA1 Monoclonal antibody, PBS Only (Capture/Detector)
- 抗体名:
IBA1 Monoclonal antibody, PBS Only (Capture/Detector)
- 规格:
100ug
产品信息
66827-5-PBS targets IBA1 as part of a matched antibody pair:
MP51023-2: 66827-3-PBS capture and 66827-5-PBS detection (validated in Cytometric bead array)
MP51023-3: 66827-5-PBS capture and 66827-4-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag28236 Product name: Recombinant human IBA1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-147 aa of BC009474 Sequence: MSQTRDLQGGKAFGLLKAQQEERLDEINKQFLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKAREKEKPTGPPAKKAISELP |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | allograft inflammatory factor 1 |
| 别名 | AIF1, G1, allograft inflammatory factor 1, AIF-1, AIF 1 |
| 计算分子量 | 17 kDa |
| GenBank蛋白编号 | BC009474 |
| 基因名称 | IBA1 |
| Gene ID (NCBI) | 199 |
| ENSEMBL Gene ID | ENSG00000204472 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G Magarose purification |
| UNIPROT ID | P55008 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验a. 制备活性较好的细胞悬液,调节浓度至107个/ml。 b. 在小试管(5×50mm)中加入100ul细胞悬液,再加入100ul待检McAb的样品,混匀,4℃作用30-90分钟。 c. 用洗涤液(PBS 900ml,小牛血清50ml,4%NaN3 50ml)洗二次,每次加洗涤液1-5ml,1000r/min离心5分钟,弃上清。加入100ul荧光抗体,4℃作用30-90分钟。 d. 同法洗涤,将细胞重新悬于20-30ul含10%甘油和10-100ug苯二胺/ml
; 2、一抗用多克隆抗体易出现非特异性着色,建议用单克隆抗体看看; 3、内源性过氧化物酶和生物素在肝脏、肾脏等组织含量很高(含血细胞多的组织),需要通过延长灭活时间和增加灭活剂浓度来降低背景染色; 4、非特异性组分与抗体结合,这需要通过延长二抗来源的动物免疫血清封闭时间和适当增加浓度来加强封闭效果; 5、缩短 DAB 孵育时间或降低 DAB 浓度/过氧化氢浓度等; 6、适当增加 PBS 冲洗次数和浸洗时间,在一抗、二抗或 SP 孵育之后的浸洗尤为重要; 7、防止标本染色过程中
Detection of Viruses in Infected Plant Extracts using Immunocapture-PCR
(1Xworking conc.) Incubate at 370 C for 2.5 h in moist chamber Wash 3X with PBS-Tween wash buffer (1Xworking conc.) �let air dry 2) Antigen capture steps: Grind leaf extracts 1:3 w/v in extraction buffer (1Xworking conc.)
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