- ￥5800
- Proteintech
- 84621-3-PBS
- 2025年10月09日
- WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA
- Rabbit
- human
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- 详细信息
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA
- 靶点：
SPARC
- 抗体英文名：
SPARC Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名：
SPARC Recombinant monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
84621-3-PBS targets SPARC as part of a matched antibody pair:
MP01430-2: 84621-1-PBS capture and 84621-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg1169 Product name: Recombinant Human SPARC protein (His Tag) (HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-10*His Domain: 18-303 aa of NM_003118.4 Sequence: APQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | secreted protein, acidic, cysteine-rich (osteonectin) |
| 别名 | 241952E11, Basement membrane protein 40, Basement-membrane protein 40, BM 40, BM-40 |
| 计算分子量 | 35kDa |
| 观测分子量 | 40-43 kDa |
| GenBank蛋白编号 | NM_003118.4 |
| 基因名称 | SPARC |
| Gene ID (NCBI) | 6678 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P09486 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).
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