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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human Cytochrome c protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 750 Fluorescent Dye
- 适应物种:
human, mouse
- 宿主:
Rabbit
- 应用范围:
WB, FC (Intra)
- 靶点:
Cytochrome c
- 抗体英文名:
CoraLite® Plus 750-conjugated Cytochrome c Recombinant monoclonal antibody
- 抗体名:
CoraLite® Plus 750-conjugated Cytochrome c Recombinant monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive WB detected in | Jurkat cells, HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-83276 targets Cytochrome c in WB, FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, FC (Intra) |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag1455 Product name: Recombinant human Cytochrome c protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-105 aa of BC009578 Sequence: MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cytochrome c, somatic |
| 别名 | CYCS, CYC, 230503E10 |
| 计算分子量 | 12 kDa |
| 观测分子量 | 12-15 kDa |
| GenBank蛋白编号 | BC009578 |
| 基因名称 | Cytochrome c |
| Gene ID (NCBI) | 54205 |
| RRID | AB_3673778 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P99999 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.
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文献和实验Screening Colonies by Hybridization with Radiolabeled Probe
and any (random or specific) primers or with templates within a vector and specific primers. Supplies & Equipment: Prime It® II Random Primer Labeling Kit (Stratagene #300385) Waterbath 37°C Waterbath 100°C Microfuge
体内后,通过糖苷键结合参与代谢,转换为葡萄糖醛酸轭合物或硫酸轭合物。所以β-受体激动剂的检测通常需要酶解步骤,β-葡萄糖醛酸酶是一种可以使葡萄糖醛酸苷键加水分解的酶,它可以把葡萄糖醛酸轭合物或硫酸轭合物的糖苷键切断,释放出游离态。目前,β-受体激动剂类药物残留的检测方法包括高效液相色谱法(HPLC)、气相色谱-质谱法(GC-MS)、液相色谱-质谱/质谱法(LC-MS/MS)、毛细管电泳电气化学检测法。下面介绍使用新型重组酶 IMCSzyme® β-葡萄糖醛酸酶与 MicroSep® DPX 枪头式分散固相
体 对于包涵体的纯化,包涵体的前处理是很重要的。 包涵体中主要含有重组蛋白,但也含有一些细菌成分,如一些外膜蛋白、质粒DNA和其它杂质。洗涤常用1%以下的中性去垢剂,如Tween、Triton、Lubel和 NP40等加EDTA和还原剂2-巯基苏糖醇(DTT)、β-巯基乙醇等反复多次进行,因去垢剂洗涤能力随溶液离子强度升高而加强,在洗涤包涵体时可加50 mM NaCL。这样提取的包涵体纯度至少可达50%以上,而且可保持元结构。也可用低浓度的盐酸胍或尿素/中性去垢剂/EDTA/还原剂等洗去
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