- ￥3200
- Proteintech
- CL750-16216
- 2025年10月09日
- IF-P, FC (Intra)
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human HBB protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
human, mouse
- 宿主：
Rabbit
- 应用范围：
IF-P, FC (Intra)
- 靶点：
HBB
- 抗体英文名：
CoraLite® Plus 750-conjugated HBB Polyclonal antibody
- 抗体名：
CoraLite® Plus 750-conjugated HBB Polyclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF-P detected in | human appendicitis tissue, human placenta tissue |
| Positive FC (Intra) detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-16216 targets HBB in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF-P, FC (Intra) |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag9216 Product name: Recombinant human HBB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC007075 Sequence: MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hemoglobin, beta |
| 别名 | beta globin, Beta-globin, Hemoglobin beta chain, Hemoglobin subunit beta, LVV-hemorphin-7 |
| 计算分子量 | 147 aa, 16 kDa |
| 观测分子量 | 13-16 kDa |
| GenBank蛋白编号 | BC007075 |
| 基因名称 | HBB |
| Gene ID (NCBI) | 3043 |
| RRID | AB_3673690 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P68871 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.
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文献和实验Screening Colonies by Hybridization with Radiolabeled Probe
dH2 O) Denaturing solution (0.5 N NaOH, 1.5 M NaCl) (dissolve 43.83 g NaCl in 300 ml dH2 O, add 50 ml 10 N NaOH stock solution , dH2 O ad 500 ml) Neutralizing solution (1.5 M NaCl, 0.5 M Tris ・ Cl [pH 7.4]) (100 ml dH
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