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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
PerCP-Cyanine5.5 Fluorescent Dye
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
FC
- 靶点:
CD8b
- 抗体英文名:
PerCP-Cyanine5.5 Anti-Human CD8b Rabbit Recombinant Antibody
- 抗体名:
PerCP-Cyanine5.5 Anti-Human CD8b Rabbit Recombinant Antibody
- 规格:
25tests/100tests
| 规格: | 25tests | 产品价格: | ¥900.0 |
|---|---|---|---|
| 规格: | 100tests | 产品价格: | ¥1800.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CPY5-98103 targets CD8b in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD8b molecule |
| 别名 | CD8, 241326A7, CD8b molecule, CD8B1, Leu2 |
| 计算分子量 | 243 aa, 27 kDa |
| GenBank蛋白编号 | BC100913 |
| 基因名称 | CD8B |
| Gene ID (NCBI) | 926 |
| 偶联类型 | PerCP-Cyanine5.5 Fluorescent Dye |
| 最大激发/发射波长 | 482 nm / 690nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P10966 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on the surface of cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
-277): sc-7148 SOURCE Caspase-3 (H-277) is a rabbit polyclonal antibody raised against a recombinant protein corresponding to amino acids 1-277 representing the full length precursor form of caspase-3 of human origin. SPECIFICITY Caspase-3 (H-277
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