- ￥5800
- Proteintech
- 65103-1-PBS
- 2025年10月09日
- FC, Sandwich ELISA, Indirect ELISA, Sample test
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
FC, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
CD40
- 抗体英文名：
Anti-Human CD40 (G28.5), PBS Only (Detector)
- 抗体名：
Anti-Human CD40 (G28.5), PBS Only (Detector)
- 规格：
100ug
产品信息
65103-1-PBS targets CD40 as part of a matched antibody pair:
MP50064-1: 66965-2-PBS capture and 65103-1-PBS detection (validated in Sandwich ELISA)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | FC, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD40 molecule, TNF receptor superfamily member 5 |
| 别名 | B cell surface antigen CD40, B-cell surface antigen CD40, Bp50, CD40L receptor, G28.5 |
| 计算分子量 | 277 aa, 31 kDa |
| GenBank蛋白编号 | BC012419 |
| 基因名称 | CD40 |
| Gene ID (NCBI) | 958 |
| ENSEMBL Gene ID | ENSG00000101017 |
| RRID | AB_2881471 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P25942 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).
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文献和实验in immunoassays. In respect to surface blocking, different types of nonspecific interactions can show up as increased background. A common problem is that the detector antibody can nonspecifically bind either to a non-optimally blocked surface or, in case
FACS Procedures for Apoptosis Detection
Materials: Hoechst 33258 (Sigma B-2883). stock: 10 mg/ml in dH20 (40 ) working dilution: 500µg/ml (50µl stock + 950µl PBS). 7-Amino-actinomycin (Sigma A-9400) stock: 200µg/ml in dH
antibody at 5 µg/ml for 45 min at 4 °C. Mouse anti-human NFB p65 monoclonal antibody sc-8008 (Santa Cruz, CA, USA) recognizes epitopes mapping the C amino-acid terminus of human origin NFB p65. After two washes in PBS–FCS, nuclei were incubated for 45 min
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