- ￥800 - 1990
- Proteintech
- CL750-65050
- 2025年10月09日
- FC (Intra)
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
Mouse
- 宿主：
Rat
- 应用范围：
FC (Intra)
- 靶点：
CD107a / LAMP1
- 抗体英文名：
CoraLite® Plus 750 Anti-Mouse CD107a / LAMP1 (1D4B)
- 抗体名：
CoraLite® Plus 750 Anti-Mouse CD107a / LAMP1 (1D4B)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥800.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1990.0 |
经过测试的应用
| Positive FC (Intra) detected in | NIH/3T3 cells |
| Positive FC detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.5 ug per 10^6 cells in 100 μl suspension |
| Flow Cytometry (FC) | FC : |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65050 targets CD107a / LAMP1 in FC (Intra) applications and shows reactivity with Mouse samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | Mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | lysosomal-associated membrane protein 1 |
| 别名 | AI196048, CD107a, Lamp 1, Lamp1 |
| GenBank蛋白编号 | BC006785 |
| 基因名称 | CD107a |
| Gene ID (NCBI) | 16783 |
| RRID | AB_3673695 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P11438 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
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文献和实验20B淋巴细胞表面的膜蛋白CD20对B淋巴细胞的增殖和分化具有调节作用[1]。由于CD20分子仅在前-B淋巴细胞、未成熟B淋巴细胞、成熟B淋巴细胞、激活B淋巴细胞中表达，而在浆细胞、淋巴多能干细胞以及其它组织均无CD20的表达[2，3，4]，在人体血清中亦无游离CD20的存在[5]，因此CD20可作为B淋巴细胞瘤治疗的一个理想治疗靶点。限制抗体临床使用的因素主要有抗体产生的免疫原性和抗体的生产能力是否能够满足临床需要。第四届国际人类白细胞分化抗原会议将鼠源性单克隆抗CD20抗体HI47（IgG3）正式命名为CD20+X
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
Homo sapiens matrix metalloproteinase-like 1 (MMPL1), mRNA 695 NM_001950 Homo sapiens E2F transcription factor 4, p107/p130-binding (E2F4), mRNA 956 NM_003815 Homo sapiens a disintegrin and metalloproteinase domain 15 (metargidin) (ADAM15), mRNA
经750g、离心10min后留上清，以9000g离心20min后留沉淀，重新悬浮后以9000g再离心20min，弃上清得线粒体.全部操作于4℃进行。 (2)培养细胞提取线粒体。细胞弃去培养液后，用细胞收集液作用2到3分钟，用scraper，将细胞收集起来，6000g，离心15分钟。弃上清，之后加细胞匀浆液，用匀浆器或手动匀浆管匀浆，将装有匀浆液的离心管配平后放入普通离心机，以2500rpm，离心15分钟，缓缓取上清液，移入高速离心管中，保存于有冰块的烧杯中。以17000rpm离心20分钟，弃上清
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