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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1, kappa
- 保存条件:
Store at 2-8°C. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Rat
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
CD43
- 抗体英文名:
Anti-Rat CD43 (W3/13)
- 抗体名:
Anti-Rat CD43 (W3/13)
- 规格:
100ug/500ug
| 规格: | 100ug | 产品价格: | ¥1260.0 |
|---|---|---|---|
| 规格: | 500ug | 产品价格: | ¥3870.0 |
经过测试的应用
| Positive FC detected in | Wistar rat splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 1 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
65237-1-Ig targets CD43 in FC applications and shows reactivity with Rat samples.
| 经测试应用 | FC |
| 经测试反应性 | Rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | sialophorin |
| 别名 | Cd43, gpL115, Lsn, Lsn1, sialophorin, Spn |
| 计算分子量 | 38 kDa |
| 基因名称 | CD43 |
| Gene ID (NCBI) | 24796 |
| RRID | AB_2935148 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P13838 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
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文献和实验to be an anti-adhesive molecule mediating repulsion between leukocytes and other cells, although it may act as an adhesion molecule under other circumstances Expression defective in Wiskott-Aldrich syndrome, although secondary to X linked genetic defect
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Ususally 1:1000 (v/v) in PBS. Wash in PBS 2' x3. Equilibrate slides in Buffer 1, RT x 5'. Incubate slides in Blocking Buffer, RT x 30' Incubate with anti-Dig-AP conjugate (1:2000 in Blocking buffer.), 2 hrs. RT, or o.n. at 4ºC.
which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
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