- ￥2800
- Proteintech
- CL488-81377
- 2025年10月09日
- IF/ICC, FC (Intra)
- Rabbit
- human, mouse, rat
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human EEF1A1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
EEF1A1
- 抗体英文名：
CoraLite® Plus 488-conjugated EEF1A1 Recombinant monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated EEF1A1 Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-81377 targets EEF1A1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1938 Product name: Recombinant human EEF1A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 144-462 aa of BC014224 Sequence: GVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation elongation factor 1 alpha 1 |
| 别名 | 2B16, CCS 3, CCS3, EC:3.6.5.-, EEF 1 |
| 计算分子量 | 462 aa, 50 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC014224 |
| 基因名称 | EEF1A1 |
| Gene ID (NCBI) | 1915 |
| RRID | AB_3084501 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P68104 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell proliferation and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved.
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