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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2b, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
CoraLite® Plus 750 Fluorescent Dye
- 适应物种:
Mouse
- 宿主:
Rat
- 应用范围:
FC
- 靶点:
Ly-6G/Ly-6C (Gr-1)
- 抗体英文名:
CoraLite® Plus 750 Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5)
- 抗体名:
CoraLite® Plus 750 Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5)
- 规格:
25ug/100ug
| 规格: | 25ug | 产品价格: | ¥800.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥1990.0 |
经过测试的应用
| Positive FC detected in | mouse bone marrow cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65140 targets Ly-6G/Ly-6C (Gr-1) in FC applications and shows reactivity with Mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | Mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | lymphocyte antigen 6 complex, locus G |
| 别名 | Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G (Gr-1) |
| GenBank蛋白编号 | X70920 |
| 基因名称 | Ly-6G |
| Gene ID (NCBI) | 546644 |
| RRID | AB_2935104 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P35461 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).
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文献和实验Intracellular Cytokine Staining Protocol
14 Sa2-8 14-0141 not on blood monocytes Granulocytes Ly6G, GR-1 RB6-8C5 14-5931 blood neutrophils, BM granulocytes and myeloid lineage
Intracellular Cytokine Staining Protocol
14 Sa2-8 14-0141 not on blood monocytes Granulocytes Ly6G, GR-1 RB6-8C5 14-5931 blood neutrophils, BM granulocytes and myeloid lineage
of m e m b r an e-b o u n d B ak -B cl-x L p r o te in sin vitro. Protein Eng. Des. SeL 1 7 , 501 -508.
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