- ￥5800
- Proteintech
- 82821-6-PBS
- 2025年10月09日
- WB, Dot Blot, Indirect ELISA, ChIP-qPCR
- Rabbit
- human, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, rat
- 宿主：
Rabbit
- 应用范围：
WB, Dot Blot, Indirect ELISA, ChIP-qPCR
- 靶点：
Mono-Methyl-Histone H3 (Lys9)
- 抗体英文名：
Mono-Methyl-Histone H3 (Lys9) Recombinant monoclonal antibody, PBS Only
- 抗体名：
Mono-Methyl-Histone H3 (Lys9) Recombinant monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
82821-6-PBS targets Mono-Methyl-Histone H3 (Lys9) in WB, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, Dot Blot, Indirect ELISA, ChIP-qPCR |
| 经测试反应性 | human, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H3a |
| 别名 | dmk9, H3 Me Lys9, H3 MeK9, H3 Methyl K9, H3 Methyl Lys9 |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC066245 |
| 基因名称 | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P68431 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression.
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文献和实验Reagents 100-bp DNA size ladder (Promega) Agarose Antisera (affinity-purified) These should be raised against histone peptides with mono-, di-, or trimethylation or acetylation at a specific lysine/arginine residue
Isolation of Histones and Nucleosome Cores from Mammalian Cells
is often important for understanding mechanisms of regulation of transcription and other processes that occur on DNA. The basic unit of chromatin is the nucleosome core, containing two copies each of the core histones H2A, H2B, H3, and H4 to form a histone octamer
解开双链DNA，产生单/低聚核小体片段，而核小体本身由于DNA与组蛋白H1、H2A、H2B、H3和H4形成紧密复合物而不被核酸内切酶裂解。采用双抗体夹心酶免疫法，应用小鼠抗DNA和抗组蛋白的单克隆抗体，与核小体片段形成夹心结构，可特异性检测细胞溶解物中的单/低聚核小体，从而判断细胞是否发生凋亡。 【材料与试剂】 （1） 生物素标记的小鼠抗-组蛋白单克隆抗体； （2） 过氧化物酶（-POD）标记的小鼠抗DNA单克隆抗体； （3） 链霉亲合素包被的微孔板； （4） DNA-组蛋白复合
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