- ￥5800
- Proteintech
- 84474-5-PBS
- 2025年10月09日
- WB, IHC, IF/ICC, Indirect ELISA
- Rabbit
- mouse
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, Indirect ELISA
- 靶点：
CD107b / LAMP2
- 抗体英文名：
Mouse CD107b / LAMP2 Recombinant monoclonal antibody, PBS Only
- 抗体名：
Mouse CD107b / LAMP2 Recombinant monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
84474-5-PBS targets CD107b / LAMP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, Indirect ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2029 Product name: Recombinant Mouse CD107b/LAMP2 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 26-379 aa of NM_001017959.2 Sequence: LIVNLTDSKGTCLYAEWEMNFTITYETTNQTNKTITIAVPDKATHDGSSCGDDRNSAKIMIQFGFAVSWAVNFTKEASHYSIHDIVLSYNTSDSTVFPGAVAKGVHTVKNPENFKVPLDVIFKCNSVLTYNLTPVVQKYWGIHLQAFVQNGTVSKNEQVCEEDQTPTTVAPIIHTTAPSTTTTLTPTSTPTPTPTPTPTVGNYSIRNGNTTCLLATMGLQLNITEEKVPFIFNINPATTNFTGSCQPQSAQLRLNNSQIKYLDFIFAVKNEKRFYLKEVNVYMYLANGSAFNISNKNLSFWDAPLGSSYMCNKEQVLSVSRAFQINTFNLKVQPFNVTKGQYSTAQDCSADEDN |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | lysosomal-associated membrane protein 2 |
| 别名 | CD107b, Lamp2, 241893A7, Lamp 2, Mac3 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 90-110 kDa |
| GenBank蛋白编号 | NM_001017959.2 |
| 基因名称 | CD107b / LAMP2 |
| Gene ID (NCBI) | 16784 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P17047-1 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验。加入甘氨酸可以消除甲醛使交联反应终止。1.1.准备两个长满细胞的150cm2的细胞培养皿（1*107-5*107 个细胞/皿）。将甲醛直接滴入PBS洗过的细胞培养皿中，使其终浓度为0.75%，然后在室温缓慢旋转10分钟，使蛋白和DNA发生交联。1.2 加入甘氨酸使其终浓度为125mM,在室温晃动孵育5分钟。1.3 使用10ml预冷PBS清洗细胞2次1.4 使用细胞刮将细胞收获放入 5ml 预冷PBS中，并转入50ml的管子。1.5.在皿里加入3mlPBS，将剩余的细胞转移到50ml管子里1000g
the Swiss Webster or CD1 outbred strains, although past usage includes both C57Bl/6 and B6C3F1. The Anesthetic Avertin (2,2,2 tribromoethanol) is found to be quite effective. For method of preparation, see "Manipulating the Mouse Embryo", CSHL
of the total. Red cell count: Dilute cells 1/1000 in PBS. Add 10 µL to a hemocytometer. Count the number of RBC per large square. Calculate: RBC/large square x 1,000 dilution x 10 large squares/µL = RBC/µL blood. White cell count: Add 10 µL
技术资料暂无技术资料 索取技术资料
