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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human IRE1; ERN1 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
WB, ELISA
- 靶点:
IRE1/ERN1
- 抗体英文名:
IRE1/ERN1 Polyclonal antibody
- 抗体名:
IRE1/ERN1 Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28164-1-AP targets IRE1/ERN1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag27375 Product name: Recombinant human IRE1; ERN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 233-444 aa of BC130405 Sequence: QREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDM |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | endoplasmic reticulum to nucleus signaling 1 |
| 别名 | ERN1, IRE1; ERN1, Endoplasmic reticulum-to-nucleus signaling 1, Endoribonuclease, ERN 1 |
| 计算分子量 | 977 aa, 110 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC130405 |
| 基因名称 | IRE1 |
| Gene ID (NCBI) | 2081 |
| RRID | AB_3086033 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75460 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | J Inflamm Res A Neutrophil Extracellular Traps-Related Gene Trait Revealed the Prospective Therapy Strategy of Coronary AtherosclerosisAuthors - Zhetao Li |
因此,综合来说,EarthOx的GST标签抗体 (货号#E022040)是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时,我们也提供其他品牌的GST标签抗体,如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody(货号#10000-0-AP)以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体,请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
Cell cycle phase Mammalian Yeast G0/G1 AP-1 family (FOS, JUN)
同时,Apaf―1可以激活原形的caspase―9,形成细胞色素C/Apaf―1/caspase―9复合体,即凋亡复合体(apoptosome)。该复合体水解并激活下游的caspase通路如caspase―3等。 凋亡复合体的形成是凋亡信号启动的关键步骤。目前发现激活的癌基因可以辅助凋亡复合体的形成,比如腺病毒EIA可以上调Apaf―1和caspase―9,E2F1可以上调Apaf―1的表达。Apaf―1的释放可促进凋亡复合体的形成,对凋亡的启动起促进作用。目前的研究认为,细胞
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