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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Cardinal Red™ Fluorescent Dye
- 适应物种:
Human
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
CD38
- 抗体英文名:
Cardinal Red™ Anti-Human CD38 (HIT2)
- 抗体名:
Cardinal Red™ Anti-Human CD38 (HIT2)
- 规格:
25tests/100tests
| 规格: | 25tests | 产品价格: | ¥920.0 |
|---|---|---|---|
| 规格: | 100tests | 产品价格: | ¥2310.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CR-65111 targets CD38 in FC applications and shows reactivity with Human samples.
| 经测试应用 | FC |
| 经测试反应性 | Human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD38 molecule |
| 别名 | ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1 |
| 计算分子量 | 300 aa, 34 kDa |
| GenBank蛋白编号 | BC007964 |
| 基因名称 | CD38 |
| Gene ID (NCBI) | 952 |
| ENSEMBL Gene ID | ENSG00000004468 |
| RRID | AB_3673831 |
| 偶联类型 | Cardinal Red™ Fluorescent Dye |
| 最大激发/发射波长 | 592 nm / 611 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P28907 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).
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剂,重悬在流式染色缓冲液或者 PBS 里面)。如果需要长时间保存样本,建议在样本做完表面染色和固定后,保存在专门的保存缓冲液中。但即便是有专门的保存 buffer,对于用了串联染料(如 APC/Cy7、PE/Cy7 等)染色的样本,我们还是建议不要长时间保存,很难避免补偿的「漂移(shift)」。左图:human PBMC 刺激完当天染色后上机检测;右图:同一样本,表染和固定后,保存在 Cyto-Last™ buffer 里面,14 天后做胞内染色并上机。Ki- 67 染色时,选择哪种固定方式?流式
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