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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human Cystatin C protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 488 Fluorescent Dye
- 适应物种:
Human, mouse
- 宿主:
Rabbit
- 应用范围:
IF-P
- 靶点:
Cystatin C
- 抗体英文名:
CoraLite® Plus 488-conjugated Cystatin C Recombinant monoclonal antibody
- 抗体名:
CoraLite® Plus 488-conjugated Cystatin C Recombinant monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF-P detected in | mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82441 targets Cystatin C in IF-P applications and shows reactivity with Human, mouse samples.
| 经测试应用 | IF-P |
| 经测试反应性 | Human, mouse |
| 免疫原 |
CatNo: Ag2890 Product name: Recombinant human Cystatin C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-146 aa of BC013083 Sequence: AGSSPGKPPRLVGGPMDASVEEEGVRRALDFAVGEYNKASNDMYHSRALQVVRARKQIVAGVNYFLDVELGRTTCTKTQPNLDNCPFHDQPHLKRKAFCSFQIYAVPWQGTMTLSKSTCQDA |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cystatin C |
| 别名 | ARMD11, CST3, Cystatin 3, cystatin C, Gamma trace, Post gamma globulin |
| 计算分子量 | 146 aa, 16 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC013083 |
| 基因名称 | Cystatin C |
| Gene ID (NCBI) | 1471 |
| RRID | AB_3673064 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01034 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.
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,于室温处理 1 小时进行终止。+4°C 环境下,将神经元细胞与神经元标志微管相关蛋白 2 (microtubule-associated protein 2,MAP-2) (英国剑桥Abcam,ab11267, HM-2,1:600 稀释)小鼠单克隆抗体共同孵育,孵育过夜。第 2 天,将细胞与山羊抗小鼠二抗(Alexa Fluor® 488 goat anti-mouse IgG (H+L) (Invitrogen,1:250 稀释)共同孵育。使用DMI6000 B 倒置显微镜(德国,Leica
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