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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
WB, Indirect ELISA
- 靶点:
Prion protein PrP/CD230
- 抗体英文名:
Prion protein PrP/CD230 Recombinant monoclonal antibody, PBS Only
- 抗体名:
Prion protein PrP/CD230 Recombinant monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
85611-4-PBS targets Prion protein PrP/CD230 in WB, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg3359 Product name: Recombinant Human Prion protein PrP protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 23-229 aa of BC012844 Sequence: KKRPKPGGWNTGGSRYPGQGSPGGNRYPPQGGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQGGGTHSQWNKPSKPKTNMKHMAGAAAAGAVVGGLGGYMLGSAMSRPIIHFGSDYEDRYYRENMHRYPNQVYYRPMDEYSNQNNFVHDCVNITIKQHTVTTTTKGENFTETDVKMMERVVEQMCITQYERESQAYYQRG |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | prion protein |
| 别名 | Prion protein PrP, PRNP, PrP, Alternative prion protein, AltPrP |
| 观测分子量 | 20-35 kDa |
| GenBank蛋白编号 | BC012844 |
| 基因名称 | PrP |
| Gene ID (NCBI) | 5621 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04156 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Prion protein (PRNP) is a ubiquitous membrane glycoprotein whose abnormal self-replicating, misfolded form is widely believed to cause several central nervous system disorders, collectively known as Transmissible Spongiform Encephalopathies (TSE). Prion diseases are TSEs, attributed to conformational conversion of the cellular prion protein (PrPC) into an abnormal conformer that accumulates in the brain. The two isoforms, PrPC and PrPS, have the same primary amino acid sequence and only differ in conformation. While PrPC is composed of 42% α-helix and only 3% β-sheet, PrPSc is composed of 30% α-helix and 43% β-sheet. PrPC converts to its pathogenic isoform when the region corresponding to the residues 108-144 fold into β-sheets. PrPC is very soluble in detergents and easily digested by proteases while the PrPSc is insoluble in detergents and resistant to protease digestion. Prion diseases exist in infectious, sporadic, and genetic forms.
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文献和实验少可以参考文献上给定的硫酸铵固体的溶解度表,《Guide to Protein Purification》一书中就有详细的溶解度表,静置两个小时左右,10000 g离心半个小时后,弃去上清,用PBS重新溶解沉淀,然后再次加入硫酸铵固体至45%饱和,静置两个小时左右10000 g离心半个小时,弃去上清,用少量PBS溶解沉淀,即得到较纯的抗体,因为此抗体中尚有较高浓度的硫酸铵,因此需要对PBS透析,一般透析一天一夜较合适,透析过程中更换透析液两至三次。如果对纯度要求不是很高,也可以不做第一次沉淀,直接用45
经严格测试,说明上所标明的溶解液是能够将该细胞因子或重组蛋白完全溶解的液体。如果您所用的溶解液的pH值和离子强度与说明书中所标明的不符,很多时候会造成细胞因子或重组蛋白不能完全溶解或者根本无法溶解,这样所配得的细胞因子或重组蛋白必然活性不够或丧失。 有不少用户没注意说明书上的描述,而是根据习惯,直接用PBS或培养液(1640或DMEM等)等溶解细胞因子或蛋白的冻干粉。这样做可以吗? 答:有时可以,要看具体情况。PeproTech的大多数细胞因子或蛋白冻干粉的溶解液不是PBS
因子或重组蛋白不能完全溶解或者根本无法溶解,这样所配得的细胞因子或重组蛋白必然活性不够或丧失。 有不少用户没注意说明书上的描述,而是根据习惯,直接用PBS或培养液(1640或DMEM等)等溶解细胞因子或蛋白的冻干粉。这样做可以吗? 答:有时可以,要看具体情况。PeproTech的大多数细胞因子或蛋白冻干粉的溶解液不是PBS,此时千万不能用PBS或培养液直接来溶解,具体原因在上面已经叙述过。而有部分细胞因子,如重组人KGF(产品编号:100-19)和重组人FGF-23(产品编号:100
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