- ￥1500 - 3280
- Proteintech
- 85763-4-RR
- 2025年10月09日
- WB, IF/ICC, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human DDX23 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 宿主：
Rabbit
- 应用范围：
WB, IF/ICC, ELISA
- 靶点：
DDX23
- 抗体英文名：
DDX23 Recombinant monoclonal antibody
- 抗体名：
DDX23 Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | COLO 320 cells, HeLa cells, HEK-293T cells, K-562 cells, A549 cells, NIH/3T3 cells, fetal human brain tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85763-4-RR targets DDX23 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0258 Product name: Recombinant human DDX23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 614-820 aa of BC002366 Sequence: ERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSESEKRKKLLAILEQGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSAVFYELKQAILESPVSSCPPELANHPDAQHKPGTILTKKRREETIFA |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 |
| 别名 | 100 kDa U5 snRNP-specific protein, DEAD box protein 23, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX23, prp28 |
| 计算分子量 | 96 kDa |
| 观测分子量 | 96-100 kDa |
| GenBank蛋白编号 | BC002366 |
| 基因名称 | DDX23 |
| Gene ID (NCBI) | 9416 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9BUQ8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 (DDX23, synonyms: prp28, MGC8416, U5-100K) is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX23 is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing.
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