- ￥800 - 1990
- Proteintech
- CL750-65219
- 2025年10月09日
- FC
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
Mouse
- 宿主：
Rat
- 应用范围：
FC
- 靶点：
CD62L
- 抗体英文名：
CoraLite® Plus 750 Anti-Mouse CD62L (MEL14)
- 抗体名：
CoraLite® Plus 750 Anti-Mouse CD62L (MEL14)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥800.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1990.0 |
经过测试的应用
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65219 targets CD62L in FC applications and shows reactivity with Mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | Mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | selectin, lymphocyte |
| 别名 | AI528707, CD62L, L selectin, LECAM 1, Lnhr, Ly 22, Ly m22, Lyam 1, Lyam1, selectin, lymphocyte, Sell |
| GenBank蛋白编号 | BC052681 |
| 基因名称 | CD62L |
| Gene ID (NCBI) | 20343 |
| RRID | AB_2935121 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P18337 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).
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文献和实验Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)
input samples may need to be extracted twice.20. Add 30 uls of 5M NaCl, 5 ugs of tRNA and 5 ugs of glycogen to each sample. Mix well then add 750 uls of ethanol. Precipitate in -200 C freezer overnight.Day 4 21. Microfuge samples at 14,000 rpm for 20
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)
to each sample. Mix well then add 750 uls of ethanol. Precipitate in -200 C freezer overnight. Day 4 21. Microfuge samples at 14,000 rpm for 20 minutes at 40 C. Allow pellets to air dry. Resuspend DNA in water or TE and analyze by PCR.
看其表达情况的话，可以直接加蛋白loading buffer煮沸5分钟即可，需注意的是上样前要离心12000rpm至少5分钟，然后上样时，枪头别吸最底下的。 4、20毫升细胞裂解液配方:40 μL 0.5M EDTA (PH8.0)，4 ml 10% SDS，1 ml 1M Tris-cl(PH6.8)，14.96 ml 蒸馏水。 5、我始终未明白楼主如果想收集全蛋白，而不考虑包涵体的话，为何要用超声呢？直接水煮不就可以了吗？ 6、将1ml菌离心弃上清，留大概50
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