- ￥800 - 1990
- Proteintech
- CL750-65088
- 2025年10月09日
- FC
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
Mouse
- 宿主：
Rat
- 应用范围：
FC
- 靶点：
CD90.2
- 抗体英文名：
CoraLite® Plus 750 Anti-Mouse CD90.2 (30-H12)
- 抗体名：
CoraLite® Plus 750 Anti-Mouse CD90.2 (30-H12)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥800.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1990.0 |
经过测试的应用
| Positive FC detected in | mouse thymocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65088 targets CD90.2 in FC applications and shows reactivity with Mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | Mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | thymus cell antigen 1, theta |
| 别名 | CD90, T25, Thy 1, Thy 1.2, Thy1, Thy1.1, Thy1.2, thymus cell antigen 1, theta |
| GenBank蛋白编号 | BC054436 |
| 基因名称 | CD90 |
| Gene ID (NCBI) | 21838 |
| RRID | AB_2935098 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P01831 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR (PMID: 5919593).
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文献和实验MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
用量（1.6 μl MHC I-Strep ＋2 μl Strep-Tactin® PE in 20 ul buffer IS） 3 倍 Streptamers 用量（2.4 μl MHC I-Strep ＋3 μl Strep-Jactin® PE in 30 μl buffer IS） 4 倍 Streptamers 用量（3.2 μl MHC I-Strep ＋4 μl Strep-Jactin® PE in 40 μl buffer IS） Streptamers 的解离
Extraction of DNA using DNAzol® Reagent
实验试剂 1. 100% ethanol 2. 75% ethanol 3. 8 N NaOH 实验步骤 Instructions for Use 1. Lysis/Homogenization 1 ml DNAzol® Reagent 25-50
E-Z 96® Viral RNA Protocol with Centrifugation
onto the microtube rack containing 1.2ml microtubes (supplied with kit). 14. Add 50-70 ul of DEPC-treated water to each well, and seal the HiBind® RNA plate with new sealing film(supplied with kit). Make sure to add water directly onto RNA matrix. Incubate
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