- ￥800 - 1990
- Proteintech
- CL750-65076
- 2025年10月09日
- FC
- Armenian Hamster
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
mouse
- 宿主：
Armenian Hamster
- 应用范围：
FC
- 靶点：
CD80
- 抗体英文名：
CoraLite® Plus 750 Anti-Mouse CD80 (16-10A1)
- 抗体名：
CoraLite® Plus 750 Anti-Mouse CD80 (16-10A1)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥800.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1990.0 |
经过测试的应用
| Positive FC detected in | mouse peritoneal macrophages |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65076 targets CD80 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Armenian Hamster / IgG |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD80 antigen |
| 别名 | Ly53, Ly 53, CD80 antigen, Cd28l, B7.1 |
| GenBank蛋白编号 | BC131959 |
| 基因名称 | CD80 |
| Gene ID (NCBI) | 12519 |
| RRID | AB_3084841 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Unknow |
| UNIPROT ID | Q00609 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)
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文献和实验Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)
1.2 mM EDTA 16.7 mM Tris-Cl pH 8.1 167 mM NaCl 1X Dialysis buffer 2 mM EDTA 50 mM Tris-Cl pH 8.0 0.2 % Sarkosyl (omit for monoclonal antibodies) IP Wash buffer 100 mM Tris-Cl pH 9.0 (8.0 for monoclonal
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)
protease inhibitors Nuclei Lysis buffer 50 mM Tris-Cl pH 8.1 10 mM EDTA 1% SDS protease inhibitors IP Dilution buffer 0.01% SDS 1.1% Trition X 100 1.2 mM EDTA 16.7 mM Tris-Cl
二、超声波处理法 1、要设定好超声时间和间隙时间，一般超声时间不超过5秒，间隙时间最好大于超声时间，这些都有利于保护酶的活性。我的实验超声时间5秒、间隙时间10秒、工作次数20次。 2、每个样本超声时间5秒,每个间隔5秒,粉碎5次。 3、100ml菌液离心，用8ml缓冲液悬浮，加8mg溶菌酶，冰浴30min，然后超声处理。750W 40％功率。5秒超声，9秒间隔。超声至少90min。 4、综合冻融和超声的方法： 1500g离心，弃除上清。冰上，用小体
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