- ￥5800
- Proteintech
- 60755-5-PBS
- 2026年05月25日
- Cytometric bead array, Indirect ELISA
- Mouse
- human
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant human IL-10RA protein
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
Cytometric bead array, Indirect ELISA
- 靶点：
IL-10RA
- 抗体英文名：
IL-10RA Monoclonal antibody, PBS Only (Capture)
- 抗体名：
IL-10RA Monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
60755-5-PBS targets IL-10RA as part of a matched antibody pair:
MP51203-4: 60755-5-PBS capture and 60755-2-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag4156 Product name: Recombinant human IL-10RA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 253-578 aa of BC028082 Sequence: CLALQLYVRRRKKLPSVLLFKKPSPFIFISQRPSPETQDTIHPLDEEAFLKVSPELKNLDLHGSTDSGFGSTKPSLQTEEPQFLLPDPHPQADRTLGNGEPPVLGDSCSSGSSNSTDSGICLQEPSLSPSTGPTWEQQVGSNSRGQDDSGIDLVQNSEGRAGDTQGGSALGHHSPPEPEVPGEEDPAAVAFQGYLRQTRCAEEKATKTGCLEEESPLTDGLGPKFGRCLVDEAGLHPPALAKGYLKQDPLEMTLASSGAPTGQWNQPTEEWSLLALSSCSDLGISDWSFAHDLAPLGCVAAPGGLLGSFNSDLVTLPLISSLQSSE |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 10 receptor, alpha |
| 别名 | |
| 计算分子量 | 578 aa, 63 kDa |
| GenBank蛋白编号 | BC028082 |
| 基因名称 | IL-10RA |
| Gene ID (NCBI) | 3587 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G Magarose purification |
| UNIPROT ID | Q13651 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验a. 制备活性较好的细胞悬液，调节浓度至107个/ml。 b. 在小试管（5×50mm）中加入100ul细胞悬液，再加入100ul待检McAb的样品，混匀，4℃作用30-90分钟。 c. 用洗涤液（PBS 900ml，小牛血清50ml，4%NaN3 50ml）洗二次，每次加洗涤液1-5ml，1000r/min离心5分钟，弃上清。加入100ul荧光抗体，4℃作用30-90分钟。 d. 同法洗涤，将细胞重新悬于20-30ul含10%甘油和10-100ug苯二胺/ml
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
analysis of integrin‐associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. Sci. Signal 2:ra51. Jankowski, A., Zhu, P., and Marshall, J.G. 2008. Capture
Dual- and Triple-Color Fluorospot
% fetal calf serum (FCS) in phosphate-buffered saline (PBS), pH 7.4. 2. Cells suspended in complete culture medium, i.e., RPMI 1640 containing 10% FCS supplemented with glutamic acid, pyruvate, HEPES, and penicillin/streptomycin. 3. Monoclonal
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