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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 宿主:
Rabbit
- 应用范围:
WB, Cytometric bead array, Indirect ELISA
- 靶点:
IL-12A/IL-12 p35
- 抗体英文名:
IL-12A/IL-12 p35 Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名:
IL-12A/IL-12 p35 Recombinant monoclonal antibody, PBS Only (Detector)
- 规格:
100ug
产品信息
85754-2-PBS targets IL-12A/IL-12 p35 as part of a matched antibody pair:
MP02056-1: 85754-3-PBS capture and 85754-2-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Eg3341 Product name: Recombinant Human IL12A protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 23-219 aa of BC104982 Sequence: RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) |
| 别名 | IL-12, CLMF, CLMF p35, IL 12 subunit p35, IL 12A |
| 计算分子量 | 25 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC104982 |
| 基因名称 | IL-12A |
| Gene ID (NCBI) | 3592 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P29459 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
IL-12A (Interleukin-12A) is a subunit of the cytokine IL-12, which is also known as IL-12 p35. IL-12 is a heterodimeric cytokine composed of two subunits: IL-12A (p35) and IL-12B (p40). This cytokine plays a crucial role in the immune response, particularly in the activation and differentiation of T cells and natural killer (NK) cells.
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文献和实验50~100μl PBS稀释,总体积为200μl。] ②荧光标记的单克隆抗体染色30min,4 ℃ (或放于冰上)。单克隆抗体的浓度因不同来源差异很大,但为了使用方便,可按其说明书配成每次实验使用10μl。注意蔽光。 ③用3~4μl冷BPS清洗。离心250×g(约每分钟1500转),5min,洗3次。注意每次用吸管将上清吸出,决不能倾倒去液! ④用2ml氯化铵液(配法见下)在室温下溶解红血球,约10min。若红细胞完全被溶解
培养基中。冰冻全血与PBMCs:使用1×红细胞裂解液处理活化的外周血或者PBMCs,用PBS漂洗,并用含1%的BSA及10%的DMSO的PBS重悬,-70℃冻存。溶化后细胞置于染色管中,加上2~3mL的洗液,离心5分钟后,用破膜剂对细胞进行破膜和染色。四、所需试剂1、细胞表面染色的荧光标记的单抗试剂依据实验需要选择特殊表面标志CD45圈定所有淋巴细胞CD3 圈定T淋巴细胞CD4 圈定T辅助淋巴细胞亚群CD8 圈定T抑制淋巴细胞亚群CD19/或CD20圈定B淋巴细胞CD56圈定NK淋巴细胞CD
血或者PBMCs,用PBS漂洗,并用含1%的BSA及10%的DMSO的PBS重悬,-70℃冻存。溶化后细胞置于染色管中,加上2~3mL的洗液,离心5分钟后,用破膜剂对细胞进行破膜和染色。 四、所需试剂 1、细胞表面染色的荧光标记的单抗试剂 依据实验需要选择特殊表面标志 CD45圈定所有淋巴细胞 CD3 圈定T淋巴细胞 CD4 圈定T辅助淋巴细胞亚群 CD8 圈定T抑制淋巴细胞亚群 CD19/或CD20圈定B淋巴细胞
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