相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
rat
- 宿主:
Rabbit
- 应用范围:
WB, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点:
IL-4
- 抗体英文名:
Rat IL-4 Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名:
Rat IL-4 Recombinant monoclonal antibody, PBS Only (Detector)
- 规格:
100ug
产品信息
84422-5-PBS targets IL-4 as part of a matched antibody pair:
MP01293-4: 84422-3-PBS capture and 84422-5-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | rat |
| 免疫原 |
CatNo: Eg1113 Product name: Recombinant Rat IL-4 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 25-147 aa of NM_201270.1 Sequence: CNDSPLREIINTLNQVTEKGTPCTEMFVPDVLTATRNTTENELICRASRVLRKFYFPRDVPPCLKNKSGVLGELRKLCRGVSGLNSLRSCTVNESTLTTLKDFLESLKSILRGKYLQSCTSMS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 4 |
| 别名 | 241249H7, B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1 |
| 计算分子量 | 16kDa |
| GenBank蛋白编号 | NM_201270.1 |
| 基因名称 | IL-4 |
| Gene ID (NCBI) | 287287 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P20096 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Interleukin-4 (IL-4) is a pleiotropic cytokine that plays a crucial role in regulating immune responses and mediating type-2 immune reactions. It is primarily produced by T-helper 2 (Th2) cells, mast cells, eosinophils, basophils, and other immune cells. IL-4 exerts its effects by binding to type I and type II receptors, initiating a signaling cascade that involves the phosphorylation of STAT6 and the transcription of IL-4-responsive genes. This pathway is essential for the regulation of immune cell functions and the maintenance of immune homeostasis.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验。 复溶后的细胞因子,4℃可稳定储存 2周;长期保存请分装后存放于-20℃以下,可稳定保存至少三个月。最好在-80℃保存。分装时浓度要>100ng/ul,体积要>20ul。 十三、重组细胞因子的交叉活性 · 大多数人类细胞因子对小鼠细胞都有活性。 · 许多小鼠细胞因子对人类细胞也有活力表现,但是比相应人类细胞因子活力低。 · 一些人类的细胞因子如IL-7在小鼠细胞中表现出比小鼠细胞因子更高的比活力。 · 干扰素、GM-CSF、IL-3 和IL-4 有物种
-2方法2 (4-24 小时)人IL-4方法4人IL-5方法1人IL-6单核细胞:方法3 (6-12 小时) T细胞:方法6人IL-10方法1人IL-12方法8人IL-15方法3人Fractalkine/CX3CL1方法1人IL-8/CXCL8方法3 (24 小时)人MCP-1/CCL2方法3 (24 小时)人MIP-1a/CCL3方法3 (24 小时)人MIP-1b/CCL4方法3 (24 小时)人RANTES/CCL5方法1小鼠IL-2方法9小鼠IL-4方法10小鼠IL-5方法10小鼠IL-6方法
细胞因子的合成,如TH1(IL-2,IFN-)与TH2(IL-4,IL-5,IL-10),但这些研究很难外推,因为T细胞克隆">克隆与体内T细胞功能相关性还未被揭示。 近来,Jung与Picker采用了monensin、PMA等药物预孵,用Brefeldin(BFA)、Monensin阻断了胞内高尔基体介导的转运的方法使得细胞因子聚集,蓄积,增强细胞因子信号可被流式细胞仪 检测。因为自然状态下,T淋巴细胞产生少量的细胞因子,通常要对T淋巴细胞体外活化进行研究。在体外刺激过程中,T
技术资料暂无技术资料 索取技术资料









