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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human CD31 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 488 Fluorescent Dye
- 适应物种:
human, mouse
- 宿主:
Rabbit
- 应用范围:
IF/ICC
- 靶点:
CD31
- 抗体英文名:
CoraLite® Plus 488-conjugated CD31 Recombinant monoclonal antibody
- 抗体名:
CoraLite® Plus 488-conjugated CD31 Recombinant monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF/ICC detected in | HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-80530 targets CD31 in IF/ICC applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag1787 Product name: Recombinant human CD31 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 428-738 aa of BC022512 Sequence: EVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKANKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKGLIAVVIIGVIIALLIIAAKCYFLRKAKAKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVGNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | platelet/endothelial cell adhesion molecule |
| 别名 | PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM |
| 计算分子量 | 83 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC022512 |
| 基因名称 | CD31 |
| Gene ID (NCBI) | 5175 |
| ENSEMBL Gene ID | ENSG00000261371 |
| RRID | AB_3673033 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P16284 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | IF | Funct Integr Genomics Narciclasine attenuates sepsis-associated acute kidney injury through the ESR1/S100A11 axisAuthors - Liping Yin |
有好几个同仁发消息问CD31染色,刚工作比较忙,今天分享一下我做的是免疫荧光,也试做过免疫组化,都可以做出来抗体:小鼠抗CD31单克隆抗体 英国Abcam公司(CD31抗体说明书里面有说多聚甲醛会掩盖抗体,所以推荐速冻切片,也尝试过速冻切片,不使用多聚甲醛,但是这个技术个人掌握不好,所以还是用了多聚甲醛固定)二抗: 山羊抗小鼠488单克隆抗体 美国CST公司 特别说明:我是从同学那里得到的经验,可能绿光稍微好做些,之前做红光
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色(可选) 1. 将细胞重悬在 0.5 ml DNA 染色液中(例如 DRAQ5® #4084)。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗: 抗兔: Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4412 Anti-Rabbit IgG(H+L), F(ab
增殖及计数分析 02 细胞鉴定和免疫分型 荧光标记的CD抗体(Abs)被广泛用于流式细胞术进行细胞识别和免疫分型,至少有15种人类T细胞亚型被描述出来(例如,CD4+,CD8+等)。Incucyte®利用相同的原理,对标记方法进行改进: 将抗CD抗体结合到同型匹配的带有荧光标签的Fc区域靶向Fab片段,便可通过简单的一步混合来完成标记。在检测中使用一种非干扰性的背景荧光抑制染料,以改善488 nm的荧光信号,保护细胞的同时提供更灵活的分析方案。 03 细胞激活和分化 免疫
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