- 询价
- Proteintech
- 65109-1-PBS
- 2025年10月09日
- IF/ICC, FC
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
IF/ICC, FC
- 靶点：
CD45
- 抗体英文名：
Anti-Human CD45 (HI30), PBS Only
- 抗体名：
Anti-Human CD45 (HI30), PBS Only
- 规格：
None
产品信息
65109-1-PBS targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, C |
| 别名 | EC:3.1.3.48, GP180, HI30, L CA, LCA |
| GenBank蛋白编号 | BC014239 |
| 基因名称 | CD45 |
| Gene ID (NCBI) | 5788 |
| ENSEMBL Gene ID | ENSG00000081237 |
| RRID | AB_2918407 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P08575 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
1 cells are used to generate T-lineage cells, and OP9 cells are used to generate B-lineage and myelo-erythroid cells. OP9 medium Phosphate-buffered saline (PBS; Hyclone SH30256) Recombinant human Flt-3/Flk-2 ligand (R&D Systems 308-FK
MATERIALS T-cell: CD2 IgG2b, 01171D B-cell: B220 (CD45R) IgG2a, 01121A CD3 IgG2b, 28001D Monocytic: Mac1 (CD11b) IgG2b, 01711A CD5 IgG2a, 01031
血清） （must be the same species as primary antibody）。This control is easy to achieve and can be used routinely in immunohistochemical staining.这个可以咨询试剂商。同型对照为免疫荧光标记中的阴性对照。由于荧光标记单抗的组来源不同，应选用相同来源的未标记单抗作为同型对照来调整背景染色。举个例子：比如检测一抗为单抗的mouse anti rat CD11b，clone OX-42 purified IgG
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