FcZero-rAb™ CoraLite® Plus 488
Rabbit IgG Isotype Control Recombinant Antibody CL488-FcA98136- ￥750 - 1500
- Proteintech
- CL488-FcA98136
- 2025年10月09日
- FC
- Rabbit
- n/a
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
n/a
- 宿主：
Rabbit
- 应用范围：
FC
- 靶点：
IgG Isotype Control
- 抗体英文名：
FcZero-rAb™ CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody
- 抗体名：
FcZero-rAb™ CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥750.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1500.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-FcA98136 targets IgG Isotype Control in FC applications and shows reactivity with n/a samples.
| 经测试应用 | FC |
| 经测试反应性 | n/a |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | IgG Isotype Control |
| 别名 | 240953C9, Rabbit IgG, Rabbit IgG Control, Rabbit IgG Isotype, Rabbit IgG Isotype Control |
| 基因名称 | |
| Gene ID (NCBI) | |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
as described earlier (25 ). Briefly, mouse IgG was non-covalently immobilized on the sample slide, or rabbit IgG on the control slide. Slides were covered with 20 μg/ml IgG solutions in 50 mM Na-phosphate buffer (pH 7.3) and incubated at room temperature
as many as 6 million dpm to maximize sensitivity. 4) Put the 100,000 dpm samples aside until step #12. 5) After lyophilization, solubilize 200,000 dpm samples in 350 µl IMP buffer. 6) Add 50 µl of antibody or control (normal rabbit serum, mouse isotype control
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