- ￥5800
- Proteintech
- 83041-1-PBS
- 2025年10月09日
- WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR
- 靶点：
Acetyl-Histone H2A (Lys9)
- 抗体英文名：
Acetyl-Histone H2A (Lys9) Recombinant monoclonal antibody, PBS Only
- 抗体名：
Acetyl-Histone H2A (Lys9) Recombinant monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
83041-1-PBS targets Acetyl-Histone H2A (Lys9) in WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR |
| 经测试反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H2ae |
| 别名 | Ac Histone H2A, Ac Histone H2A (Lys9), Ac Histone H2A Lys9, Ac Histone H2A-Lys9, Acetyl Histone |
| 计算分子量 | 14 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC093836 |
| 基因名称 | HIST1H2AE |
| Gene ID (NCBI) | 3012 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04908 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Histone H2A is a core component of nucleosome. Histone variants contribute to chromatin complexity by creating specialized nucleosomes. Within nucleosomes, either one canonical H2A or both of them can be exchanged with a particular variant (heterotypic and homotypic nucleosomes, respectively), and such changes can have profound influences on nucleosome stability and biological outcome.
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文献和实验Isolation of Histones and Nucleosome Cores from Mammalian Cells
is often important for understanding mechanisms of regulation of transcription and other processes that occur on DNA. The basic unit of chromatin is the nucleosome core, containing two copies each of the core histones H2A, H2B, H3, and H4 to form a histone octamer
解开双链DNA，产生单/低聚核小体片段，而核小体本身由于DNA与组蛋白H1、H2A、H2B、H3和H4形成紧密复合物而不被核酸内切酶裂解。采用双抗体夹心酶免疫法，应用小鼠抗DNA和抗组蛋白的单克隆抗体，与核小体片段形成夹心结构，可特异性检测细胞溶解物中的单/低聚核小体，从而判断细胞是否发生凋亡。 【材料与试剂】 （1） 生物素标记的小鼠抗-组蛋白单克隆抗体； （2） 过氧化物酶（-POD）标记的小鼠抗DNA单克隆抗体； （3） 链霉亲合素包被的微孔板； （4） DNA-组蛋白复合
Use of In Vivo Biotinylation for Chromatin Immunoprecipitation
ratio). (A ) Silver stain of biotinylated protein expressed in HEK 293 cells, separated by SDS‐PAGE and washed with either standard buffer or 2% SDS. (B ) Interaction of histone H2A.Z with the total genome of NIH3T3 cells. NIH3T3 cells were transiently
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