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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
rat
- 宿主:
Rabbit
- 应用范围:
Cytometric bead array, Indirect ELISA
- 靶点:
Fas Ligand/TNFSF6
- 抗体英文名:
Rat Fas Ligand/TNFSF6 Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名:
Rat Fas Ligand/TNFSF6 Recombinant monoclonal antibody, PBS Only (Detector)
- 规格:
100ug
产品信息
85836-3-PBS targets Fas Ligand/TNFSF6 as part of a matched antibody pair:
MP02135-1: 85836-4-PBS capture and 85836-3-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | rat |
| 免疫原 |
CatNo: Eg3218 Product name: Recombinant Rat Fas Ligand/TNFSF6 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 103-278 aa of NM_012908.1 Sequence: HLQKELAELREFTNHSLRVSSFEKQIANPSTPSETKKPRSVAHLTGNPRSRSIPLEWEDTYGTALISGVKYKKGGLVINEAGLYFVYSKVYFRGQSCNSQPLSHKVYMRNFKYPGDLVLMEEKKLNYCTTGQIWAHSSYLGAVFNLTVADHLYVNISQLSLINFEESKTFFGLYKL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Fas ligand (TNF superfamily, member 6) |
| 别名 | Fas Ligand/CD178, ADAM10-processed FasL form, APL, Apt1Lg1, CD178 |
| 计算分子量 | 31 kDa |
| GenBank蛋白编号 | NM_012908.1 |
| 基因名称 | Faslg |
| Gene ID (NCBI) | 25385 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P36940 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验Analysis of Oligosaccharide Ligands by High Perfor
. Monitor the eluant with a UV detector (280 nm). 4. Increasing absorbance at 280 mm indicates saturation of the column. At this time switch back to the PBS buffer. 5. The amount of IgG coupled to the column is obtained by subtracting the unbound IgG
Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography
of approximately 0.5 ml/min, l0�. Monitor the eluant with a UV detector (280 nm). 4. Increasing absorbance at 280 mm indicates saturation of the column. At this time switch back to the PBS buffer. 5. The amount of IgG coupled to the column is obtained
至 1 升。室温保存。 2. 甲醛溶液(无甲醇的)。 3. 孵育缓冲液:将 0.5 g 牛血清白蛋白(bovine serum albumin,BSA)溶解在 100 ml 的 1 X PBS 中。保存在 4°C。 B. 固定 1. 离心收集细胞,弃上清。 2. 将细胞重悬在 0.5-1 ml PBS 中。加入甲醛溶液至终浓度为 2-4%。 3. 37°C 固定 10 分钟。 4. 放在冰上降温 1 分钟。 5. 做细胞外染色不需要细胞膜打孔时,继续 D1
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