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- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 488 Fluorescent Dye
- 适应物种:
human, mouse
- 宿主:
Rabbit
- 应用范围:
IF/ICC, FC (Intra)
- 靶点:
Caspase 3/P17/19
- 抗体英文名:
CoraLite® Plus 488-conjugated Caspase 3/P17/19 Recombinant monoclonal antibody
- 抗体名:
CoraLite® Plus 488-conjugated Caspase 3/P17/19 Recombinant monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82202 targets Caspase 3/P17/19 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | caspase 3, apoptosis-related cysteine peptidase |
| 别名 | CASP3, Caspase3, Caspase 3, CASP-3, CASP 3 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 32-35 kDa, 17 kDa, 19 kDa |
| GenBank蛋白编号 | NM_004346 |
| 基因名称 | Caspase 3 |
| Gene ID (NCBI) | 836 |
| RRID | AB_3084511 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P42574 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.
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antibody unconjugated; 货号21337,产品描述:GAPDH Antibody;规格 50 ul / 100 ul; 货号3052,产品描述:Rabbit anti-Human IgG secondary antibody HRP conjugated; 货号300904050,产品描述:One-Hour Western kit,10 ml / bottle x 3;
) 双阳性细胞即为幼稚红细胞,但无法区分原始及中晚幼红. 19、请问流式检测膜蛋白表达的变化用多克隆抗体出结果的机会大吗? 流式的抗体和WB的抗体是不同的(有部分可以通用),多抗一般是大的变性蛋白为抗原制备的,而WB的中被检测蛋白就是变性状态,所以很吻合,而流失中一般蛋白还是保持了天然构象,所以产生的多抗可能不太好,而需要用很小特定的决定簇为抗原制备单克隆抗体。20、在下最近作人外周血流式,作表面及其胞内染色,试剂说明书说要先分离外周血单个核细胞再染色,我有时候分的红细胞比较多,其他的方法试过
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