- ￥2600
- Proteintech
- 68345-1-PBS
- 2025年10月09日
- WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA
- Mouse
- human, mouse, rat, pig, rabbit, canine, chicken, zebrafish, hamster, dog, wheat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, pig, rabbit, canine, chicken, zebrafish, hamster, dog, wheat
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA
- 靶点：
Histone H3
- 抗体英文名：
Histone H3 Monoclonal antibody, PBS Only (Capture)
- 抗体名：
Histone H3 Monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
68345-1-PBS targets Histone H3 as part of a matched antibody pair:
MP50178-1: 68345-1-PBS capture and 66863-1-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human, mouse, rat, pig, rabbit, canine, chicken, zebrafish, hamster, dog, wheat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H3a |
| 别名 | HIST1H3A, 1A2A3, Histone H 3, HistoneH3, Histone-H3 |
| 观测分子量 | 15-17 kDa |
| GenBank蛋白编号 | BC066245 |
| 基因名称 | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P68431 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.
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文献和实验upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
should also be included. In Ringrose et al ., (2004) I used histone H3 peptides spanning residues 1-13 (for lysine 4 and lysine 9 modifications) or 21-33 (for lysine 27 modifications). Stock solutions of 0.5mM peptide in PBS can be prepared and kept at -20°C
upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
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