- ￥5800
- Proteintech
- 66911-1-PBS
- 2025年10月09日
- WB, IHC, Cytometric bead array, Indirect ELISA
- Mouse
- human
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human MAVS; VISA protein
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
WB, IHC, Cytometric bead array, Indirect ELISA
- 靶点：
MAVS; VISA
- 抗体英文名：
MAVS; VISA Monoclonal antibody, PBS Only (Capture)
- 抗体名：
MAVS; VISA Monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
66911-1-PBS targets MAVS; VISA as part of a matched antibody pair:
MP50509-3: 66911-1-PBS capture and 66911-4-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IHC, Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag5949 Product name: Recombinant human MAVS; VISA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-347 aa of BC044952 Sequence: MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYESYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEKDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPS |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mitochondrial antiviral signaling protein |
| 别名 | MAVS, 1A8E9, Cardif, IPS 1, IPS1 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 50-55 kDa, 70-75 kDa |
| GenBank蛋白编号 | BC044952 |
| 基因名称 | MAVS |
| Gene ID (NCBI) | 57506 |
| RRID | AB_2882238 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q7Z434 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验ELISPOT（Enzyme－linked Immunospot Assay）全名为酶联免疫斑点检测，结合了细胞培养技术与酶联免疫吸附技术，能够检测到单个细胞分泌的细胞因子情况。简单来说，就是用包被好的抗体捕获培养中的细胞分泌的细胞因子，并以酶联斑点显色的方式将其呈现出来。ELISPOT 实验原理ELISPOT 实验使用 PVDF 膜为底的 96 孔板（cat：MSIPS 4510，Millipore），包被上特异性的单克隆抗体（需要无毒，不含内毒素，亲和力高的抗体），以捕获细胞分泌的细胞
% NaCl将细菌溶液稀释至一定浓度。 2. 加50μl于微孔板内，4℃过夜。同时设阴性对照（加50μl 0.85% NaCl于另一孔内）。 3. 用400μl的0.05% 温20pBS（以下简称TPBS），洗涤五次。 4. 加入2.25%的100μl正常山羊血清(NGS) PBS封闭2小时. 5. 用含0.15% NGS的TPBS洗3次. 6. 加入100μl用0.75% NGS适当稀释的单克隆抗体(内含100μg的鲜鱼精DNA),室温孵育30
技术资料暂无技术资料 索取技术资料
