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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Cardinal Red™ Fluorescent Dye
- 适应物种:
n/a
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
IgG1 Isotype Control
- 抗体英文名:
Cardinal Red™ Mouse IgG1 Isotype Control (MOPC-21)
- 抗体名:
Cardinal Red™ Mouse IgG1 Isotype Control (MOPC-21)
- 规格:
25ug/100ug
| 规格: | 25ug | 产品价格: | ¥700.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥1750.0 |
经过测试的应用
| Positive FC detected in |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CR-65124 targets IgG1 Isotype Control in FC applications and shows reactivity with n/a samples.
| 经测试应用 | FC |
| 经测试反应性 | n/a |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin heavy constant gamma 1 (G1m marker) |
| 别名 | Ighg1, MOPC-21, Igh-4, IgG1 |
| 基因名称 | IgG1 Isotype Control |
| Gene ID (NCBI) | 16017 |
| RRID | AB_3084953 |
| 偶联类型 | Cardinal Red™ Fluorescent Dye |
| 最大激发/发射波长 | 592 nm / 611 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P01868 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.
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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse
Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
Mouse Strains and Genetic Nomenclature
. We also provide some guidance to make the best possible choice when establishing an experimental protocol. Curr. Protoc. Mouse Biol. 1:213?238. © 2011 by John Wiley & Sons, Inc. Keywords: inbred strains; congenic strains; recombinant inbred
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