- ￥2800
- Proteintech
- CL750-83112
- 2025年10月09日
- WB
- Rabbit
- human, mouse, rat
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human NEDD4 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
NEDD4
- 抗体英文名：
CoraLite® Plus 750-conjugated NEDD4 Recombinant monoclonal antibody
- 抗体名：
CoraLite® Plus 750-conjugated NEDD4 Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive WB detected in | C6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-83112 targets NEDD4 in WB applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag16291 Product name: Recombinant human NEDD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 970-1319 aa of BC152452 Sequence: TVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | neural precursor cell expressed, developmentally down-regulated 4 |
| 别名 | 230478F1, Cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, EC:2.3.2.26, HECT-type E3 ubiquitin transferase NEDD4 |
| 计算分子量 | 1319 aa, 149 kDa |
| 观测分子量 | 115 kDa |
| GenBank蛋白编号 | BC152452 |
| 基因名称 | NEDD4 |
| Gene ID (NCBI) | 4734 |
| RRID | AB_3673770 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P46934 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Screening Colonies by Hybridization with Radiolabeled Probe
dH2 O) Denaturing solution (0.5 N NaOH, 1.5 M NaCl) (dissolve 43.83 g NaCl in 300 ml dH2 O, add 50 ml 10 N NaOH stock solution , dH2 O ad 500 ml) Neutralizing solution (1.5 M NaCl, 0.5 M Tris ・ Cl [pH 7.4]) (100 ml dH
体内后，通过糖苷键结合参与代谢，转换为葡萄糖醛酸轭合物或硫酸轭合物。所以β-受体激动剂的检测通常需要酶解步骤，β-葡萄糖醛酸酶是一种可以使葡萄糖醛酸苷键加水分解的酶，它可以把葡萄糖醛酸轭合物或硫酸轭合物的糖苷键切断，释放出游离态。目前，β-受体激动剂类药物残留的检测方法包括高效液相色谱法（HPLC）、气相色谱-质谱法（GC-MS）、液相色谱-质谱/质谱法（LC-MS/MS）、毛细管电泳电气化学检测法。下面介绍使用新型重组酶 IMCSzyme® β-葡萄糖醛酸酶与 MicroSep® DPX 枪头式分散固相
体 对于包涵体的纯化，包涵体的前处理是很重要的。 包涵体中主要含有重组蛋白,但也含有一些细菌成分,如一些外膜蛋白、质粒DNA和其它杂质。洗涤常用1%以下的中性去垢剂，如Tween、Triton、Lubel和 NP40等加EDTA和还原剂2-巯基苏糖醇（DTT）、β-巯基乙醇等反复多次进行，因去垢剂洗涤能力随溶液离子强度升高而加强，在洗涤包涵体时可加50 mM NaCL。这样提取的包涵体纯度至少可达50%以上，而且可保持元结构。也可用低浓度的盐酸胍或尿素/中性去垢剂/EDTA/还原剂等洗去
技术资料暂无技术资料 索取技术资料
