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- 湖北省武汉市
- Arabidopsis thaliana 4CL1重组蛋白表达
- 2025年09月14日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：Q42524
基因名：4CL1
蛋白名：4-coumarate--CoA ligase 1
蛋白别名：
4-coumarate--CoA ligase 1, 4CL 1, EC 6.2.1.12, E, EC 6.2.1.34, 4-coumarate--CoA ligase isoform 1, At4CL1, 4-coumaroyl-CoA synthase 1
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 |
基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
4CL1相关研究文献：
1. Compensatory Guaiacyl Lignin Biosynthesis at the Expense of Syringyl Lignin in 4CL1-Knockout Poplar. [32139476]
2. Sense-, antisense- and RNAi-4CL1 regulate soluble phenolic acids, cell wall components and growth in transgenic Populus tomentosa Carr. [23434928]
3. Four Isoforms of Arabidopsis 4-Coumarate:CoA Ligase Have Overlapping yet Distinct Roles in Phenylpropanoid Metabolism. [26491147]
4. Overexpression of artificially fused bifunctional enzyme 4CL1-CCR: a method for production of secreted 4-hydroxycinnamaldehydes in Escherichia coli. [26264710]
5. Isolation and characterization of the 4-coumarate:coenzyme A ligase (4CL1) promoter from Eucalyptus camaldulensis. [27729726]
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文献和实验2. Sense-, antisense- and RNAi-4CL1 regulate soluble phenolic acids, cell wall components and growth in transgenic Populus tomentosa Carr. [23434928]
3. Four Isoforms of Arabidopsis 4-Coumarate:CoA Ligase Have Overlapping yet Distinct Roles in Phenylpropanoid Metabolism. [26491147]
4. Overexpression of artificially fused bifunctional enzyme 4CL1-CCR: a method for production of secreted 4-hydroxycinnamaldehydes in Escherichia coli. [26264710]
5. Isolation and characterization of the 4-coumarate:coenzyme A ligase (4CL1) promoter from Eucalyptus camaldulensis. [27729726]
十字花科。 二年生草本，高 7～ 40厘米。基生叶有柄呈莲座状，叶片倒卵形或匙形；茎生叶无柄，披针形或线形。总状花序顶生，花瓣 4片，白色，匙形。长角果线形，长 1～ 1.5厘米。花期 3～ 5月。我国内蒙、新疆、陕西、甘肃、西藏、山东、江苏、安徽、湖北、四川、云南等省区均有发现。拟南芥的优点是植株小（ 1平方厘米可种植好几棵）、每代时间短（从发芽到开花不超过 6周）、结子多（每棵植物可产很多粒种子）、生活力强（用普通培养基就可作人工培养）。拟南芥的基因组是目前已知植物
Growth of Other Species Related to Arabidopsis thaliana
Recent interest in the study of biological phenomena in Brassicaceae members (crucifers) related to the model plant, Arabidopsis thaliana , fuels the need for standard protocols for growth and maintenance of these plant species
Identifying Photoprotection Mutants in Arabidopsis thaliana
of enzymes, which convert H2 O2 into H2 O. Two of the genes encoding APX in Arabidopsis are induced by high light, namely APX1 and APX2. We have applied a genetic approach to understanding the mechanisms of photoprotection, using APX2 as an indicator
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