- 询价
- Signalway Antibody
- 57048
- 2026年01月23日
- WB IHC ICC/IF
- Recombinant Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Semaphorin 4D / CD100 Rabbit mAb
- 抗体英文名：
Semaphorin 4D / CD100 Rabbit mAb
- 应用范围：
WB IHC ICC/IF
- 宿主：
Recombinant Rabbit
- 适应物种：
Human
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 保存条件：
Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
- 亚型：
Rabbit IgG
- 免疫原：
A synthesized peptide derived from human Semaphorin 4D / CD100
purification
Affinity-chromatography
applications
WB IHC ICC/IF
appl_detail
WB:1:500~1:2000
IHC:1:50~1:200
ICC/IF:1:50~1:200
other_names
BB18; CD100; COLL4; Collapsin 4; GR3; MSEMA; Sema4d; Semacl2; SemaH; SEMAJ; Semaphorin 4D; Semaphorin H; Semaphorin J; Semcl2;
calculated_mw
120,140kDa
formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
uniprot
Q92854
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文献和实验T-Cell Activation Using mAb to CD3
gives a good response. If experimental conditions require, a titration of cell densities (2-3x106 /ml to 105 /ml) should be performed for optimization. Add 100µl of the cell suspension to each well. For each condition, use triplicate wells.
1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight.(2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00)(3) 50 mM Tris pH7.8.(4) 40 g ammonium sulfate for every 100 ml Sup.(5) 4L 100 mM Tris/pH
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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