- 询价
- Signalway Antibody
- 57048
- 2025年09月22日
- WB IHC ICC/IF
- Recombinant Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthesized peptide derived from human Semaphorin 4D / CD100
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB IHC ICC/IF
- 靶点：
Uniprot：Q92854
- 抗体英文名：
Semaphorin 4D / CD100 Rabbit mAb
- 规格：
50ul/100ul
计算分子量:120,140kDa
配方:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:WB:1:500~1:2000
IHC:1:50~1:200
ICC/IF:1:50~1:200
图片:
Western blot analysis of Semaphorin 4D / CD100 expression in SH-SY5Y cell lysate.
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文献和实验T-Cell Activation Using mAb to CD3
gives a good response. If experimental conditions require, a titration of cell densities (2-3x106 /ml to 105 /ml) should be performed for optimization. Add 100µl of the cell suspension to each well. For each condition, use triplicate wells.
1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight.(2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00)(3) 50 mM Tris pH7.8.(4) 40 g ammonium sulfate for every 100 ml Sup.(5) 4L 100 mM Tris/pH
Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00) 50 mM Tris pH7.8 40 g ammonium sulfate for every 100 ml
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