alpha sarcoglycan Conjugated A

LABELING ALPHA-ACTININ WITH IATR

of injection buffer)and reading OD555. D/P = {OD555 x 41 / 60,000} / {(mg/ml)/ 100,000} 13.Dilute to 5 mg/ml for microinjection if necessary.Extra conjugated alpha-actinin can be stored as aliquots in liquid N2.

In situ hybridisation to alpha satellite sequences (chromosome specific)

  Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA

Western Blot Analysis of Epitoped-tagged Proteins Using The Chemifluorescent Detection Method - for alkaline phosphatase conjugated antibodies

0.1% Tween-20 and 0.2% I-Block), incubate 30 min, room temperature, gently rocking. Wash three times in 20 ml 1X Western buffer (containing 0.1% Tween-20 and 0.2% I-Block) for 5 min each. Add appropriate dilution of secondary antibody conjugated