DYKDDDDK Tag (FLAG) Conjugated

Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions

Figure 19.20.1 The Strep/FLAG tandem affinity purification. (A ) N‐ and C‐terminal SF‐TAP tags (POI, protein of interest). (B ) Overview of both purification steps. (1) Purification by the tandem Strep‐tag II moiety: binding to Strep‐Tactin matrix

除了分离细胞，磁珠的这些用法你了解吗？

Tag Isolation Kits 实现 2 个小时内从细胞到纯化蛋白的过程，整个过程不需离心、无背景、无材料损失，且兼容自动化高通量的提取流程。基本原理及工作流程如下： μMACS Tag Isolation Kits 支持带有如 HA, His, FLAG (DYKDDDDK), c-myc, GFP, GST 等多种标签肽段表达的目的蛋白纯化。其高灵敏度和无背景的纯化效果，在真核细胞蛋白表达系统的纯化过程中优势明显。 （左）兼容全自动高通量蛋白提取纯化平台 （右）裂解 107 个转导

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure