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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Myosin 10 Rabbit mAb
- 抗体英文名:
Myosin 10 Rabbit mAb
- 应用范围:
WB
- 宿主:
Recombinant Rabbit
- 适应物种:
Human;Mouse;Rat
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 保存条件:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 亚型:
IgG
- 免疫原:
A synthetic peptide of human non-muscle Myosin IIB/MYH10
purification
Affinity Purified
applications
WB
appl_detail
WB: 1/1000
other_names
NMMHCB; NMMHC-IIB
calculated_mw
Calculated MW:229 kDa,Observed MW:229 kDa
formulation
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
uniprot
P35580
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文献和实验7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
Myosin Light Chain Preparation
) For Cardiac Muscle- (1.5 liters ETOH 95% (1.5 liters H2O,3 ml 15 M MCE) Buffer E.Column Buffer: 16 liters: 20 mM Tris,15 mM MCE (160 ml 2 M Tris,16 ml 15 M MCE) Buffer F.Elution Buffer: 4 liters: 0.150 M KCl,10 mM Tris,15 mM MCE(150 ml 4 M KCl,40 ml 2 M
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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