- 询价
- Signalway Antibody
- 52814
- 2026年01月23日
- WB IHC
- Recombinant Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
alpha Skeletal Muscle Actin Rabbit mAb
- 抗体英文名：
alpha Skeletal Muscle Actin Rabbit mAb
- 应用范围：
WB IHC
- 宿主：
Recombinant Rabbit
- 适应物种：
Human;Mouse;Rat
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 保存条件：
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 亚型：
IgG
- 免疫原：
A synthetic peptide of human muscle Actin
purification
Affinity Purified
applications
WB IHC
appl_detail
WB: 1/1000; IHC: 1/50;
other_names
ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
calculated_mw
Calculated MW:42 kDa,Observed MW:42 kDa
formulation
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
uniprot
P68133
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文献和实验Preparation of Conventional Actin from Skeletal Muscle
Modified from Spudich & Watt, 1971, JBC 246:4866. 1. Mix 20 ml buffer G with each gm of muscle acetone powder. Extract with stirring on ice for 30 min. Use a big stir bar or overhead stirrer. Buffer G: 0.2 mM ATP, 0.5 mM DTT, 0.2 mM
the column with 3 l of Buffer D using a fast flow rate. 2. Elute the column with a 4 l gradient from 100 mM KCl to 500 mM KCl in Buffer D. Collect 25 ml fractions (400 drops/fraction). Actin-containing fractions are identified by dot blot using mAb C4
Protein purification: skeletal muscle myosin
consists of an alpha-helical coiled-coil rod of 150 nm in length. The most important mechano-enzyme for muscle contraction. Material Source: rabbit skeletal muscle
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