- ￥4749.50
- RayBio
- 107-10017
- 2025年07月14日
- Western blot
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Anti-alpha Actin (skeletal muscle) Antibody
- 抗体英文名：
Anti-alpha Actin (skeletal muscle) Antibody
- 浓度：
0.11 mg/ml
- 应用范围：
Western blot
- 宿主：
Rabbit
- 克隆性：
/
- 保存条件：
-20°C
- 亚型：
/
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文献和实验Preparation of Conventional Actin from Skeletal Muscle
Modified from Spudich & Watt, 1971, JBC 246:4866. 1. Mix 20 ml buffer G with each gm of muscle acetone powder. Extract with stirring on ice for 30 min. Use a big stir bar or overhead stirrer. Buffer G: 0.2 mM ATP, 0.5 mM DTT, 0.2 mM
by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
LABELING MUSCLE ACTIN WITH IATR
,small vial. Procedure (perform under reduced light,4℃ unless otherwise noted) 1.Resuspend 10 mg lyophilized actin in 2 ml buffer 1.Be careful not to make bubbles. 2.Add DTT (100 mM stock)to 5 mM. 3.Dialyze against 250 ml buffer 1 overnight
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