- 询价
- Signalway Antibody
- 52715
- 2026年01月23日
- WB
- Recombinant Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Actin Related Protein 2 Rabbit mAb
- 抗体英文名：
Actin Related Protein 2 Rabbit mAb
- 应用范围：
WB
- 宿主：
Recombinant Rabbit
- 适应物种：
Human;Mouse;Rat
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 保存条件：
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 亚型：
IgG
- 免疫原：
A synthetic peptide of human Arp2
purification
Affinity Purified
applications
WB
appl_detail
WB: 1/1000
other_names
ARP2
calculated_mw
Calculated MW:45 kDa,Observed MW:45 kDa
formulation
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
uniprot
P61160
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文献和实验Protein purification; actin Overview ACTIN The most abundant muscle and non-muscle cytoskeletal protein. MW 42 kDa, 374/375 amino acids; various isoforms; structure resolved at 2.8 Angstrom. [see also protocol ID 207,208,209]
David Amberg Dialyze purified GST fusion proteins and actin into PBS + 1mM MgCl2 . Mix 5ug actin into 50ul total volume binding buffer. Mix 5ug GST-fusion protein into total volume 50ul binding buffer.
by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
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