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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
CDC25 Rabbit mAb
- 抗体英文名:
CDC25 Rabbit mAb
- 应用范围:
WB
- 宿主:
Recombinant Rabbit
- 适应物种:
Human;Rat
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 保存条件:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 亚型:
Rabbit IgG
- 免疫原:
A synthetic peptide of human CDC25
purification
Affinity Purified
applications
WB
appl_detail
WB: 1/1000-1/5000
other_names
GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
calculated_mw
Calculated MW: 145 kDa; Observed MW: 145 kDa
formulation
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
uniprot
Q13972
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文献和实验fractions in 5 ml tubes with 700 ul 1 M Tris pH 9.0 already in bottom of tube to neutralize (collect at ~5 min/fraction).25 tubes are sufficient for collection. In general, you can see high Ab concentrations in 7-8 tubes.Test 1 ul on pH paper.Read OD to know
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
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