- 询价
- Signalway Antibody
- C92823APC
- 2025年09月22日
- IF
- mouse
- Hu Rat
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Hu Rat
- 保质期：
12 months
- 抗原来源：
mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
mouse
- 应用范围：
IF
- 靶点：
Uniprot：P02771
- 抗体英文名：
AFP(A2) mouse Monoclonal Antibody APC Conjugated
- 规格：
50ul/100ul
计算分子量:65
配方:0.01M PBS(pH7.4) with 0.03% Proclin300.
应用详情:IF=1:50-200
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文献和实验Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
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