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- 详细信息
- 文献和实验
- 技术资料
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Hu Ms
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
FC;ICC;IF
- 靶点:
Uniprot:P05187
- 抗体英文名:
Placental alkaline phosphatase (PLAP) Polyclonal Antibody APC Conjugated
- 规格:
50ul/100ul
计算分子量:53
配方:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
应用详情:Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
phosphatase gene, PLAP (Kam et al 1985), is perhaps the most useful as it is very heat stable and is resistant to some chemical inhibitors that are active on other endogenous alkaline phosphatases. PLAP has been used as a histochemical reporter fairly recently
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
antibody. 30 minutes. 5. Jet wash in tris/HC1 saline buffer pH 7.6. 6. Wash in buffer. 3 minutes. Steps 7-9 for POLYCLONAL Primary Antisera Only 7. Incubate in mouse anti-rabbit immunoglobulins. 30 minutes. 8. Jet wash in buffer.
Western Blotting with Alkaline Phosphatase Conjugates
buffer (1% BSA, 10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20). Incubate the blot for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C. INCUBATION WITH ALKALINE PHOSPHATASE CONJUGATED ANTIBODIES Dilute the AP
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